Table 1.
Depth parameters, Φ, for spin labeled sites on syt1C2AB when associated with POPC:POPS (3:1) or POPC:POPS:PIP2 (74:25:1) bilayers in the presence of calcium. The distances and range were calculated for PIP2 containing bilayers. †
| Labeled Site | Φ PC: PS | Φ (PC: PS: PIP2) | Distance (Å) | Range (Å) |
|---|---|---|---|---|
| M173R1 | 0.8 ± 0.3 | 1.2 ± 0.3 | +8.8 | 8.0 < × < 9.6 |
| G174R1 | 0.6 ± 0.1 | 1.8 ± 0.2 | +10.5 | 9.9 < × < 11.0 |
| K236R1 | −0.3 ± 0.2 | 1.2 ± 0.2 | +8.8 | 8.2 < × < 9.3 |
| V283R1 | −1.9 ± 0.1 | −2.2 ± 0.1 | −10.6 | −35.0 < × < −7.3 |
| T285R1 | −1.4 ± 0.1 | −1.5 ± 0.1 | −0.6 | −1.3 < × < 0.0 |
| V304R1 | −0.4 ± 0.2 | 0.4 ± 0.3 | +6.7 | 5.8 < × < 7.5 |
| G305R1 | 0.8 ± 0.2 | −1.1 ± 0.1 | +1.6 | 1.1 < × < 2.0 |
| G306R1 | 0.3 ± 0.3 | 0.2 ± 0.1 | +6.1 | 5.8 < × < 6.4 |
| K327R1 | −2.2 ± 0.2 | −1.7 ± 0.1 | −2.1 | −3.0 < × < −1.3 |
| G368R1 | 0.6 ± 0.1 | −0.9 ± 0.1 | +2.4 | 0.6 < × < 3.9 |
| K369R1 | −1.2 ± 0.2 | 0.5 ± 0.2 | +6.9 | 6.4 < × < 7.5 |
| A395R1 | −1.0 ± 0.1 | −1.9 ± 0.1 | −4.0 | −5.4 < × < −3.0 |
| N396R1 | −2.1 ± 0.1 | −1.4 ± 0.1 | +0.0 | −0.6 < × < 0.6 |
†
Depth parameters were obtained by progressive power saturation of the EPR spectra and were used to estimate depths as described in Methods. The range was determined by uncertainty in the measured values of Φ and Eq. 1.