Table 3.

Distances and distance distributions obtained by double electron-electron resonance (DEER) between spin labeled sites in C2A and C2B^†

C2A/C2B mutant	POPC:POPS (3:1)	POPC:POPS:PIP2 (74:25:1)
R199R1 / V304R1	40±14 (Å)	39 ± 16 (Å)
R199R1 / K332R1	46+8	52 ± 11
K213R1 / V283R1	61+16	56 ± 12
K213R1 / Q353R1	56+15	53 ± 15

^†Measurements were made in the presence of Ca²⁺ (see methods). Distances were obtained by a Gaussian fit to the distance distribution using the program DEER Analysis 2009 (47). The distances represent the most populated distance and the range represents one standard deviation of the Gaussian distribution. The two shorter mutant pairs have approximately a 2 Å distance uncertainty and a 30% uncertainty in the distribution. For the two distances over 50 Å there is approximately a 4 Å distance uncertainty and a 50% uncertainty in the distribution. Data for POPC:POPS was obtained previously (23) and is shown here for comparison.