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. 2011 Apr 6;6(4):e18275. doi: 10.1371/journal.pone.0018275

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Badar et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Sensitised sheep red blood cells were incubated with human C6 deficient serum to generate C3d deposition on RBC in the absence of cell lysis. ^99mTc-rCR2 , ^99mTc-C2A or K41E ^99mTc-rCR2 were added to 2.5×10⁷ C3d⁺ cells. The percentage radioactivity bound to C3d⁺ cells was determined by gamma counting the activity associated with the cell pellet as percentage of total activity added. Error bars represent the standard error of the mean of n = 5.