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. 2011 Apr 6;6(4):e18568. doi: 10.1371/journal.pone.0018568

Figure 2. Localization of human LRRK2 in the brain of transgenic mice.

Figure 2

A, In situ hybridization with 33P-labeled antisense oligonucleotide probes specific to human LRRK2 mRNA. Autoradiographs of human LRRK2 in WT (line 249), G2019S (line 340) and R1441C (line 574) transgenic mice at 2–3 months, at the level of the olfactory bulb (Olf), striatum/cortex (Str/Ctx), hippocampus/cortex (Hip/Ctx) and cerebellum (Cb). B, Localization of LRRK2 (mouse or human), and endogenous TH or α-synuclein mRNAs for comparison in adjacent midbrain sections of 2–3 month-old WT, G2019S and R1441C LRRK2 transgenic mice. C, Confocal microscopic images of LRRK2 (mouse + human; MJFF2/c41-2 antibody) and tyrosine hydroxylase (TH) immunofluorescence in the substantia nigra of 4–5 month G2019S LRRK2 transgenic (TG) mice and their non-transgenic (NTG) littermates. Bar chart showing LRRK2+ fluorescence intensity localized within nigral TH+ dopaminergic neurons of TG and NTG mice. Bars present the mean ± SEM (n = 3 mice/genotype). *P<0.001 comparing TG and NTG as indicated. Scale bar: 25 µm (C).