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. 2011 Apr 6;6(4):e18562. doi: 10.1371/journal.pone.0018562

Figure 4. pCOX2-0.8 as a minimal COX2 promoter with maximum basal response in GC cells.

Figure 4

(A) Schematic representation of pCOX2 deletions. (B–D) Gene reporter assay in MKN45 (B), AGS (C) and WI38 (D) cell lines transiently transfected with 50 ng pCOX deletions (pCOX2-1.2; pCOX2-0.8; pCOX2-0.65 and pCOX2-0.4) and 50 ng of empty vector. In all experiments cells were transfected with 1 ng of PRL-SV40 Renilla as an internal control. Promoter activity was normalized as the ratio between firefly luciferase and Renilla luciferase units. RLU: Relative Luciferase Units. Each figure corresponds to at least three independent experiments. Statistical significance was determined through ANOVA test (* p<0.05, ** p<0.01).