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. Author manuscript; available in PMC: 2012 Aug 15.
Published in final edited form as: Int J Cancer. 2010 Dec 9;129(4):799–809. doi: 10.1002/ijc.25750

Figure 4. Effects of Gal-4 knockdown.

Figure 4

(A). Effect of gal-4 RNAi on gal-4 expression in HT-29 cells. Whole cell extracts of HT-29 cells transfected either with gal-4 RNAi3 (R) or Stealth RNAi negative control (C) were subjected to immunoblotting for gal-4 expression. (B). Cell proliferation assay. HT-29 cells were transfected either with gal-4 RNAi3 or Stealth RNAi negative control for 24 h and were subjected to MTS assay as described in the methods section. A P value <0.001 (***) was considered significant. (C). Wound healing assay. HT-29 cells were transfected either with gal-4 RNAi3 or Stealth RNAi negative control and were subjected to wound healing assay as described in the Methods section. T is the time point at which the scratch was introduced and the average initial wound width was measured and considered 100%. ** indicates P <0.01 and *** indicates P <0.001.