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. Author manuscript; available in PMC: 2012 Apr 13.
Published in final edited form as: J Am Chem Soc. 2011 Mar 18;133(14):5240–5243. doi: 10.1021/ja2011109

Figure 2.

Figure 2

Characterization of NRPSs. a) A domain activities of PacP, PacU and PacW. NE, no enzyme; DABA, diaminobutyric acid; DAP, diaminopropionate; m-Tyr, meta-Tyrosine (DL). One hundred percent relative activity for l-2,3-DAP, l-Ala and m-Tyr-dependent exchange corresponds to 282k, 70k and 141k cpm respectively. b) Autoradiograph of one SDS-PAGE gel illustrating the covalent loading of 14C-labeled substrate (highlighted in red). Enzymes and substrates used in each lane are indicated in the table (highlighted in grey).