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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Nat Nanotechnol. 2011 Feb 20;6(4):253–260. doi: 10.1038/nnano.2011.12

Figure 3. Controlling the translocation times, td, of single lipid-anchored proteins by the viscosity of the bilayer coating and distinguishing proteins by their most probable td values.

Figure 3

a, Distribution of translocation times of streptavidin. Insets: current versus time traces illustrating that td could be prolonged more with intermediate viscosity POPC bilayers (blue current traces) than with low viscosity DΔPPC bilayers (red current traces). b, Translocation of anti-biotin Fab fragments through nanopores with bilayers of intermediate viscosity (POPC) or high viscosity (~49 mol% cholesterol and 50 mol% POPC). c, Translocation of anti-biotin antibodies through a pore with a coating of intermediate viscosity (POPC). Red, blue, and green curves represent a best fit of the corresponding data to a biased diffusion first passage time model14 (equation S10 in Supplementary Section S5). All bilayers contained 0.15 – 0.4 mol% biotin-PE. See Supplementary Sections S7 and S9 for binning methods, errors of td, and measurement errors.