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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Nat Struct Mol Biol. 2011 Mar 6;18(4):507–509. doi: 10.1038/nsmb.2017

Figure 3. Nucleosome removal and mRNA production.

Figure 3

(a) Cells bearing the substitution mutant of Figure 1c, (which bears the superbinder at site −2), and wild type (WT) cells, growing in 2% glucose, were transferred to medium containing 0.1% glucose and 2% galactose. Nucleosome occupancy at the WT site −2 (yellow) and the superbinder-substituted site −2 (blue) was assayed at the indicated time points following induction. (b) Aliquots of cells used for the experiment of (a) were assayed for GAL1 mRNA using QPCR as described1.