Skip to main content
. 2011 Apr 8;88(4):482–487. doi: 10.1016/j.ajhg.2011.02.011

Figure 5.

Figure 5

LIPN Expression Analysis

(A) Quantitative RT-PCR was performed on mRNA samples extracted from keratinocyte cultures derived from a patient and a nonaffected individual (control). Results are provided as a percentage of LIPN expression in control keratinocytes ± SD.

(B) LIPN expression was assessed via Clontech tissue blot cDNA array. Expression of LIPN was normalized to that of ACTB. Results are provided as a percentage of LIPN expression in control keratinocytes ± SD. The following abbreviations are used: ESON = esophagus; S.INT = small intestine; PLACEN = placenta; TRACH = trachea; PROST = prostate; KC = keratinocytes.

(C) Total RNA was extracted from organotypic cell cultures at day 0 (green), day 7 (blue), and day 10 (red). LIPN, IVL, and KRT10 expression were compared by using quantitative RT-PCR. Results are provided as a percentage of expression at day 0.