Table 2.
Loci that Showed the Most Significant Differential Methylation by Smoking Status on the Basis of the Different Analytical Approaches Taken
| CpG Site | Gene (Chromosome) | Psmoking | p Value Ranksa |
Median (IQR) Methylationb |
||||
|---|---|---|---|---|---|---|---|---|
| Never Smokers | Former Smokers | Current Smokers | ||||||
| Order and Psmoking of Mixed Linear Regression | ||||||||
| cg03636183 | F2RL3 (19p12) | 2.68 × 10−31 | 1 | 1 | 1 | 0.95 (0.94–0.96) | 0.94 (0.90–0.95) | 0.83 (0.78–0.88) |
| cg19859270 | GPR15 (3q11.2-q13.1) | 4.89 × 10−6 | 2 | 7 | 83 | 0.93 (0.91–0.94) | 0.92 (0.89–0.94) | 0.89 (0.86–0.91) |
| cg02564523 | ORAI2 (7q22.1) | 1.62 × 10−5 | 3 | 418 | 9 | 0.21 (0.18–0.23) | 0.21 (0.18–0.28) | 0.17 (0.13–0.20) |
| cg09155905 | FNDC8 (17q12) | 2.00 × 10−5 | 4 | 8 | 138 | 0.24 (0.21–0.25) | 0.20 (0.16–0.25) | 0.19 (0.15–0.22) |
| cg09084200 | NCAPD3 (11q25) | 2.25 × 10−5 | 5 | 198 | 2 | 0.15 (0.12–0.16) | 0.13 (0.11–0.15) | 0.11 (0.09–0.14) |
| Order and Psmoking of Kruskal-Wallis Test in Females | ||||||||
| cg03636183 | F2RL3 (19p12) | 3.08 × 10−11 | 1 | 1 | 1 | 0.95 (0.94–0.97) | 0.94 (0.92–0.96) | 0.84 (0.78–0.88) |
| cg08504583 | SLAIN1 (13q22.3) | 2.16 × 10−5 | 8 | 2 | 1343 | 0.59 (0.50–0.63) | 0.64 (0.55–0.69) | 0.69 (0.64–0.75) |
| cg24833277 | FAM83A (8q24.13) | 7.33 × 10−5 | 389 | 3 | 15,610 | 0.88 (0.85–0.91) | 0.92 (0.88–0.93) | 0.92 (0.90–0.92) |
| cg21919219 | AMY1A (1p21) | 1.01 × 10−4 | 568 | 4 | 19,056 | 0.83 (0.81–0.84) | 0.85 (0.83–0.86) | 0.83 (0.82–0.84) |
| cg12251508 | RBM3 (Xp11.2) | 1.90 × 10−4 | 10,720 | 5 | 7405 | 0.49 (0.47–0.51) | 0.45 (0.41–0.48) | 0.44 (0.40–0.48) |
| Order and Psmoking of Kruskal-Wallis Test in Males | ||||||||
| cg03636183 | F2RL3 (19p12) | 5.75 × 10−9 | 1 | 1 | 1 | 0.95 (0.94–0.96) | 0.94 (0.90–0.95) | 0.82 (0.77–0.87) |
| cg09084200 | NCAPD3 (11q25) | 2.58 × 10−5 | 5 | 198 | 2 | 0.15 (0.13–0.16) | 0.13 (0.11–0.15) | 0.11 (0.10–0.14) |
| cg18329036 | CRLS1 (20p13-p12.3) | 9.52 × 10−5 | 132 | 6206 | 3 | 0.03 (0.03–0.04) | 0.04 (0.03–0.04) | 0.03 (0.02–0.03) |
| cg21939482 | C7orf70 (7p22.1) | 1.21 × 10−4 | 1854 | 15,287 | 4 | 0.02 (0.02–0.02) | 0.02 (0.02–0.02) | 0.01 (0.01–0.02) |
| cg04541293 | PTPRT (20q12-q13) | 1.34 × 10−4 | 20,878 | 1422 | 5 | 0.16 (0.13–0.18) | 0.19 (0.17–0.25) | 0.13 (0.12–0.16) |
a
Psmoking value ranks of the mixed linear regression, Kruskal-Wallis tests in females, and Kruskal-Wallis tests in males, respectively.
b
Values in rows ordered by Kruskal-Wallis tests present medians in only the females and only the males, respectively.