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(A) Graphic visualization of the 2fFCS confocal volume and the nomenclature we use to describe its orientation relative to the direction of flow. (B) Typical fitted 2fFCS correlation curves. The four curves are comprised of two autocorrelations (one from each focus) and the forward and reverse cross-correlations. The data shown are for flow measurement in the “parallel” orientation, and therefore show a maximum in the “forward” cross-correlation. Adapted from Reference 45.
