Figure 1.

Polycystin-1 is localized in aortic endothelial cilia of embryonic mouse aorta. Isolated aorta from embryonic mouse was gently perfused with PBS to remove the remaining blood on the aorta wall and to help prevent the segment from collapsing. The aorta segment was cut to a thickness of 5 μm. The segment was stained for polycystin-1 (PC1; green) and the ciliary marker acetylated α-tubulin (α-tub; red). A merged image with nuclear marker (blue) also is shown. Arrows indicate specific staining for polycystin-1 and cilia. Images were taken at a magnification of ×90. The figure at the bottom right represents the region of an aorta segment where the micrograph of the section was taken, designated by a black box.