Figure 2.

Endothelial cells express ICAM-2 and eNOS and are regulated by temperature and IFN-γ. A, Fluorescence cell-sorting study shows that cultured endothelial cells from mouse embryonic aortas are positive for the endothelial marker ICAM-2 (open curves). Cells treated with secondary mouse antibody were used as a negative control (filled curves). n = 3. B, Endothelial cells under permissive conditions at 33°C in the presence of IFN-γ express SV40 large T antigen. When these cells are grown under nonpermissive conditions at 37°C in the absence of IFN-γ for 3 to 4 days, their expression of SV40 large T antigen can be suppressed. Human kidney (HK) cells were the negative control. Actin was used as the loading control. n = 1. C, Western blot analysis confirms the expression of eNOS in differentiated cells. Cells undergoing proliferation do not show eNOS expression. Mouse embryonic brain tissue was used as a positive control. n = 3.