Figure 8.

Agonist-provoked calcium and NO signaling is independent of cilia. A, Response of individual wild-type (wt), Pkd1^null/null (Pkd1), and Tg737^orpk/orpk (Tg737) cells to 1 μmol/L acetylcholine is pseudocolored. The calcium (red) and NO (green) are superimposed. The color bar indicates the Ca²⁺ or NO level relative to the corresponding baseline level, with black and blue representing a low (lo) level and red or green denoting a higher (hi) level. B, The average cytosolic calcium ([Ca²⁺]_cyt) response to acetylcholine. C, Plots of the average cytosolic NO ([NO]_cyt) response. Acetylcholine was applied to the cells for at least 14 seconds after the baseline values of calcium and NO were obtained as indicated by arrows. All time units are seconds. n = 3.