Figure 4.

A. [ALPHA]α-Man inhibits Acanthamoeba-induced cytopathic effect. Confluent corneal epithelial cultures in 6-well plates were incubated overnight with Acanthamoebae in the presence or absence of various saccharides. The plates were then stained with Giemsa. Clear, unstained regions indicate loss of cells. Dark, stained areas indicate presence of cells. Note that epithelial cells incubated with amoebae in media alone were destroyed almost completely during overnight incubation (Media). Methyl α-mannopyranoside ([ALPHA]α-Man) markedly inhibited the amoeba-induced CPE whereas [ALPHA]α-L-fucose ([ALPHA]α-Fuc) and [BETA]β-galactose ([BETA]β-Gal) had little effect. Cont. epithelial cells incubated in media alone without amoebae. B: Zymography of culture media collected from wells shown in Panel A: (i) epithelial cells with amoeba (E+A), (ii) epithelial cells with amoeba in the presence of mannose (E+A+M), (iii) epithelial cells alone (EM), or (iv) amoeba alone (ACM).Reprinted with permission from Cao Z et al.³³).