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. Author manuscript; available in PMC: 2012 Jan 5.
Published in final edited form as: Cell Metab. 2011 Jan 5;13(1):35–43. doi: 10.1016/j.cmet.2010.12.001

Fig. 5. A hepatocyte-specific screen identifies AMP activated protein kinase (AMPK) as a putative, positive modulator of BSEP expression.

Fig. 5

A) BSEP expression was measured via relative quantitation by QPCR in primary hepatocytes exposed to standard doses of the indicated agents for 24 hours.

B) BSEP expression was measured via relative quantitation by QPCR in primary hepatocytes exposed to adenoviruses containing cDNAs representing either constitutively active AMPK alpha or dominant negative AMPK alpha for 24 hours. Statistical comparison was made between the GFP group and either the constitutively active AMPK alpha or dominant negative AMPK alpha groups.

C) BSEP expression was measured via relative quantitation by QPCR in primary hepatocytes exposed to an adenovirus containing cDNA representing dominant negative AMPK alpha and either vehicle or 1mM AICAR for 18 hours.

All gene expression data are represented as mean + SEM. Unpaired student's t-test was used for evaluation of statistical significance. One asterisk indicates p < 0.05, two asterisks p < 0.01 and three asterisks p < 0.001.