Fig. 2.

The effect of noise on Fourier reweighted SOFI images. The simulation was done with a signal to background ratio of 3 and a signal intensity of 18 counts per time bin (‘Signal’ denotes the maximum intensity of the PSF). Using these relatively weak imaging parameters it was still possible to generate SOFI images and apply Fourier reweighting to them. In the upper panel we show how the quality of the Fourier reweighted second-order AC SOFI image improves when more frames are acquired. The same holds true for the third-order SOFI image (lower panel). However, we found that the fourth-order SOFI image could not satisfactorily be generated using these imaging parameters (due to high noise content already appearing in the fourth order AC SOFI image).