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. 2011 Feb 28;2(4):739–747. doi: 10.1364/BOE.2.000739

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©2011 Optical Society of America

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.

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Fig. 1 — Schematic experimental setup used for nonlinear imaging. The red path corresponds to the fundamental excitation beam centered at 965nm, the blue path to the SHG emission and the green path to the TPEF emission. L# are lenses; GM are the galvanometric mirrors; OL is the objective lens (40x, NA = 1.3), CO is the condenser optics (NA = 1.4); F₁ ad F₂ are the band pass filters (F₁ transmittance = 330 – 670 nm and F₂ transmittance = 475 – 485 nm); and PMT are the photo multiplier tubes.