Fig. 3.

3D projections of a) TPEF signal from neurons forming the nerve ring expressing GFP (blue) and b) SHG signal from the pharyngeal region (orange) of the C. elegans nematode. c) Merged TPEF (Green) and SHG (red) images of both structures. The 3D projection is composed of 55 stacks separated 1 μm. A single XY optical section was taken in less than 2 seconds. For the SHG image an average of 10 frames was applied to improve the signal to noise ratio. All the images are 500x500 pixels