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TPEF images from different dyes in solution. All the images are 500x500 pixels. PMT voltages are Fluo 4: 819 V, Ca-Green: 757 V, DiO: 711 V, Fluorescein: 510 V, JC1: 478 V and Phantom (paint): 572 V. The field of view of all the images is of 40x40 μm.
