Fig. 5.

Correlation of ROS formation and high-order photobleaching. (a) Multiphoton images from different points of time during photobleaching. ROS concentration increased during the drop in Hoechst fluorescence. Scale bar: 8 µm. (b) Time-dependence of ROS formation in comparison to Hoechst photobleaching for three half-life periods. Each data point represents the mean ± standard deviation of at least five cells. The half-life period of photobleaching and the half-saturation value of ROS formation were approximately at the same time (yellow box).