Fig. 6.

Influence of eGFP photobleaching on the degradation of Hoechst molecules in its environment. (a) Multiphoton images of Hoechst and eGFP before and after photobleaching of eGFP. Scale bar: 16 µm. (b) Loss of relative Hoechst fluorescence intensity referred to an absolute bleaching of eGFP for different half-life periods and both repetition rates. Each data point represents the mean ± standard error of at least eight experiments. The loss of Hoechst fluorescence intensity referred to an absolute bleaching of eGFP is within the range of 50–80%.