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. 2011 Mar 18;2(4):915–926. doi: 10.1364/BOE.2.000915

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©2011 Optical Society of America

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.

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Fig. 5 — Overview of the nuclear segmentation process and the resulting Delaunay Triangulation graph. (A) Snapshot of graphic user interface of CARS image analysis software; Red ellipses are the delineated cell boundaries. The green star represents one of the user-selected points, while the green square box indicates the image patch which will be processed; intermediate results: (B) targeted nucleus; (C) watershed segmentation algorithm; (D) local thresholding; (E) Ellipse fitting; (F) final result. (G) Delaunay Triangulation on the segmented image.