AR interacts with members of the DNA licensing complex. Androgen-sensitive LNCaP prostate cancer cells express AR as well as members of the DNA licensing complex, Orc2, Cdt1, and Cdc6 (lane 1). Immunoprecipitation (lane 2 – IgG control, lane 3 – anti-AR antibody) of AR from LNCaP cells followed by western blotting reveal that Orc2, Cdt1, and Cdc6 co-immunoprecipitated with AR. These data demonstrate AR presence at pre-RCs and strongly support a role for AR in modulating DNA replication licensing to drive prostate cancer cell proliferation. Figure from Vander Griend & Isaacs (2008) (LAPC4 data not shown).