Fig. 1.

Mouse exposure protocols. a Mice were exposed to a single intratracheal (i.t.) instillation of PBS (40 μl) or GC frass (40 μg/40 μl) and BAL fluid was harvested 18 h later. Lungs were isolated and cultured for an additional 24 h in the absence of additional stimulation. b Mice were exposed to a single intratracheal instillation and lungs were harvested 24 h later for analysis by flow cytometry. c PBS- or GC frasspulsed BMDC were instilled intratracheally at day 0; 14 days later, mice received an intratracheal instillation of either PBS or GC frass. On day 17, airway measurements were performed.