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. 2010 Nov 5;3(2):167–179. doi: 10.1159/000320718

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Copyright © 2011 by S. Karger AG, Basel

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Fig. 3 — DC subsets in the lungs following mucosal exposure to PBS. Naïve mice were administered a single intratracheal instillation of PBS (40 μl) or GC frass (40 μg/40 μl) and whole lungs were isolated 24 h later. Cells were dissociated from the lungs and stained for flow cytometry analysis. a A representative flow cytometry plot showing the characterization of mDCs (CD11c⁺, CD11b⁺, Gr1^neg, CD317^neg) and pDCs (CD11c⁺, CD11b^neg, Gr1^low, CD317⁺). b Total mDCs. c Percentage of mDCs. d Total pDCs. e Percentage of pDCs. Values are means ± SEM, n = 16 mice/group; statistical analysis was performed by ANOVA (∗ p < 0.001 vs. PBS).

Fig. 3 — DC subsets in the lungs following mucosal exposure to PBS. Naïve mice were administered a single intratracheal instillation of PBS (40 μl) or GC frass (40 μg/40 μl) and whole lungs were isolated 24 h later. Cells were dissociated from the lungs and stained for flow cytometry analysis. a A representative flow cytometry plot showing the characterization of mDCs (CD11c⁺, CD11b⁺, Gr1^neg, CD317^neg) and pDCs (CD11c⁺, CD11b^neg, Gr1^low, CD317⁺). b Total mDCs. c Percentage of mDCs. d Total pDCs. e Percentage of pDCs. Values are means ± SEM, n = 16 mice/group; statistical analysis was performed by ANOVA (∗ p < 0.001 vs. PBS).