Fig. 8.

MyD88-deficient BMDCs do not respond to GC frass. BMDCs from wild-type (C57Bl/6) or MyD88-deficient mice were cultured in the presence of GM-CSF for 6 days prior to treatment with GC frass (1 μg/ml). 18 h later, cell supernatants were collected, clarified and analyzed by ELISA. In all cases, means ± SEM are reported for each chemokine in pg/ml (n = 4 experiments) and statistical analysis was performed by ANOVA. a IL-6 levels (∗ p < 0.001 vs. PBS; ⁺ p < 0.001 vs. frass). b IL-23 levels (∗ p < 0.001 vs. PBS; ⁺ p < 0.001 vs. frass). c IL-12p70 levels (∗ p < 0.001 vs. PBS; ⁺ p < 0.001 vs. frass).