Table 1.

Co-stimulatory molecule expression on pulmonary DC populations following allergen exposure

Treatment	mDC		pDC
	CD80 MFI	CD80 Freq of parent	CD80 MFI	CD80 Freq of parent
PBS	208 ± 12	4.1 ± 0.6	199 ± 21	6.1 ± 0.4
GC frass	1,128 ± 60	38.5 ± 3.3	194 ± 41	6.7 ± 0.6
p value	<0.001	<0.001	ns	ns

Treatment	mDC		pDC
	CD86 MFI	CD86 Freq of parent	CD86 MFI	CD86 Freq of parent
PBS	480 ± 3	10.6 ± 0.6	188 ± 29	7.2 ± 1.3
GC frass	1,134 ± 59	22.7 ± 1.1	361 ± 21	8.1 ± 0.2
p value	<0.001	<0.001	0.007	ns

Naive mice were administered a single intratracheal inhalation of PBS (40 μl) or GC frass (40 μg/40 μl) and whole lungs were isolated 24 h later. Cells were dissociated from the lungs, and stained for flow cytometry analysis. mDC (CDllc⁺, CDllb⁺, Grl^neg, CD317^neg) and pDC (CDllc⁺, CDllb^neg, Grl^low, CD317+) populations were analyzed for CD86 and CD80 expression. Mean fluorescent intensity (MFI) and percentage of cells expressing the co-stimulatory molecules are shown. Values are means ± SEM, n = 8 mice/group and statistical analysis was performed by ANOVA.