Table 2.
Differential cell count in BAL fluid of mice adoptively transferred with mDCs pulsed with PBS or GC frass
Total cells | Mac | Epi | Neut | Eos | Lymph | |
---|---|---|---|---|---|---|
PBS-pulsed BMDCs | 20.8 ± 3.8 | 15.3 ± 4.2 | 2.1 ± 0.6 | 2.4 ± 1.8 | 0.08 ± 0.07 | 0.9 ± 0.3 |
GC frass-pulsed BMDCs | 47.8 ± 3.7 | 13.3 ± 1.6 | 2.1 ± 0.1 | 24.4 ± 5.7 | 4.6 ± 1.7 | 3.3 ± 0.5 |
p value | 0.043 | ns | ns | 0.011 | 0.036 | 0.006 |
Naive mice were sensitized with PBS- or GC frass-pulsed BMDCs on day 0. Mice that received PBS-pulsed BMDCs were challenged with PBS (40 μl) and mice that received GC frass-pulsed BMDCs were challenged with GC frass (40 μg/40 μl) on day 14. All mice were sacrificed 72 h later, BAL fluid was harvested and differential cell counts performed. These data represent 8 mice per group and are expressed as mean ± SEM cell number X103. Statistical significance between GC frass and PBS exposure was determined by ANOVA.