Figure 2.

Competitive inhibition ELISA of SLE serum antibody from six patients (1 to 6). The inhibitors used were native human IgG and ROS-modified human IgG. The microtitre plates were coated with the native DNA (2.5 μg/ml). Varying amounts of inhibitors (0.01–20 μg/ml) were allowed to interact with a constant amount of antiserum for 2 h and overnight at 4 °C, the mixture was added to antigen-coated plates and the residual antibody level was detected by ELISA. Each histogram represents the mean ± SEM of three independent assays.