Table 1.
Observed chromatographic responses of Aristolochic acids on TLC chromatograms.
| Type of adsorbent | Solvent system | Rf value |
Appearance of spots/bands |
||
|---|---|---|---|---|---|
| Day light | UV at 365 nm | 0.5% DPA/60% H2SO4 | |||
| Silica-gel 60 F254, precoated aluminum sheets, 20 × 20 cm (Merk, Germany) | CHCL3/MeOH (6:1) | 0.43 | Yellow fluorescence | Black spot | Pale grey spot |
| Silica-gel G glass plates (s d Fine-Chem., Limited, Mumbai, India) | CHCL3/MeOH (6:1) | 0.46 | Yellow fluorescence | Faint brown spot | Pale grey spot |
| Cellulose, precoated plastic sheets, 20 × 20 cm (Macherey-Nagel, Germany) | CHCL3/MeOH/Water (6:4:1) | 0.83 | Yellow fluorescence | Faint brown spot | Pale grey spot |