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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Curr Protoc Neurosci. 2011 Apr;CHAPTER:Unit2.13. doi: 10.1002/0471142301.ns0213s55

Figure 2.13.4.

Figure 2.13.4

One tissue slice is placed into each of the 35-mm wells of the culture dish. At the time the tissue is placed into the well, the well already contains approximately 2 ml of PBS to ensure the tissue does not dry out.