Figure 1. The C-terminal acidic tail is required for HMGB1 to inhibit efferocytosis.

(A) Schematic diagram showing constructs that express 3× Flag-fused human HMGB1 and ΔC-HMGB1. The amino acid number at the end of each domain is shown. (B) Effects of full-length HMGB1 and ΔC-HMGB1 on phagocytosis of apoptotic neutrophils by peritoneal macrophages.. Phagocytosis assays were performed as described in Materials and Methods. Three additional independent experiments provided similar results; ***P<0.001.