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. 2010 Nov;88(5):863–875. doi: 10.1189/jlb.0510253

Figure 7. Ectopic expression of Fyn kinase in Fyn null MCs restores TRPC1 expression, calcium responses, cortical F-actin depolymerization, and degranulation.

Figure 7.

(A) Fyn null MCs stably transduced with Fyn kinase show normal expression of TRPC1 protein. Statistical significance relative to WT MCs is **P < 0.01. (B) Ectopic expression of Fyn kinase in Fyn null MCs restores enhanced calcium responses, which were measured as described in Materials and Methods. Data shown are mean ± se of 3 independent experiments. (C) Ectopic expression of Fyn kinase in Fyn null MCs restores degranulation to the level of WT MCs. One representative of 4 experiments is shown. Data are mean ± se. Statistical significance relative to Fyn null MCs was **P < 0.01. (D) Actin dynamics were measured in WT and Fyn kinase-transduced Fyn null MCs. The arrow indicates the time of Ag addition. Mean response (±se) is shown (19–26 cells were monitored in total).