Figure 5.

Gel-shift assay to test protein-nucleic acid binding. Proteins (type and amount as indicated) were incubated with a 40 nucleotide 5′ end-labeled RNA and run on a 6% native polyacrylamide as described in Methods. The positions of the unshifted 40-mer and shifted material are indicated. Two controls are as described below the part. Representative results are shown and the assays were repeated twice with similar results.