Skip to main content
. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: J Endocrinol. 2010 May 13;206(2):171–181. doi: 10.1677/JOE-09-0456

Figure 6.

Figure 6

Ubiquitination of IRS1 induced by HG. CHO/IR/IRS1His6 cells were incubated in DMEM–0·25% BSA containing MG132 (25 μM) and 5·5 or 25 mM glucose in the presence or absence of LiCl (20 mM) for 24 h. After treatments, His6-tagged IRS1 (IRS1His6) was purified using Ni-NTA beads and detected by immunoblotting with ubiquitin antibody (FK2) and IRS1 antibody respectively. Results are representative of two separate experiments.