Fig. 2.
Whole-animal weights, cholesterol pools, and synthesis rates in the various knockout animals. All mice in these groups were males, 49 days of age. A: Animal weights at the time the studies were undertaken in the Galgt1+/+/Siat9+/+ (n = 11), Galgt1−/−/Siat9+/+ (n = 6), Galgt1+/+/Siat9−/− (n = 10), and Galgt1−/−/Siat9−/− (n = 6) mice. B: Plasma cholesterol and triacylglycerol levels in these animals. After determining the cholesterol content of all tissues in the body, the values were summed and normalized to a body weight of 1.0 kg. These values are presented in C as milligrams of cholesterol present in the whole animal per kilogram body weight. Similarly, the rates of synthesis in all organs were summed and normalized to a 1.0 kg body weight. These 3H2O incorporation rates were then converted to milligrams of cholesterol synthesized so that the rates of whole-animal cholesterol synthesis in D represent the milligrams of sterol newly synthesized per day per kilogram body weight. The asterisk (*) identifies those values in the knockout animals that were significantly different (P < 0.05) from the respective values seen in the control Galgt1+/+/Siat9+/+ mice. Each column represents the mean ± SEM.