Table 1.
Summary of crystallographic data and refinement
| Data collection | 70S-EF-Tu trp-tRNATrp (merged from 5 crystals) |
70S-EF-Tu G24A trp- tRNATrpUGG (from 2 crystals) |
70S-EF-Tu G24A trp- tRNATrpUGA (from 2 crystals) |
70S-EF-Tu A9C trp- tRNATrpUGA (from 2 crystals) |
|---|---|---|---|---|
| Space Group | P21 | P21 | P21 | P21 |
| Cell dimensions | ||||
| a, b, c (Å) |
a=290.2 b=269.2 c=404.0 |
a=289.8 b=269.1 c=404.0 |
a=289.9 b=269.4 c=404.5 |
a=289.9 b=268.5 c=403.6 |
| α, β, γ (°) | α=90.0 β=91.5 γ=90.0 |
α=90.0 β=91.2 γ=90.0 |
α=90.0 β=91.5 γ=90.0 |
α=90.0 β=91.6 γ=90.0 |
| Resolution (Å) | 50-3.1 (3.2-3.1) * | 50-3.1 (3.2-3.1) † | 50-3.1 (3.2-3.1) § | 50-3.1 (3.2-3.1) ‡ |
| Rsym | 19.9 (115.2) | 20.4 (100.9) | 26.8 (130.1) | 21.5 (103.3) |
| I/σI | 7.12 (1.24) * | 7.27 (1.35) † | 6.19 (1.23) § | 10.48 (1.69) ‡ |
| Completeness (%) |
99.8 (99.9) | 97.4 (92.7) | 98.7 (98.5) | 97.9 (93.2) |
| Redundancy | 6.3 (5.2) | 4.6 (4.1) | 6.4 (6.5) | 5.0 (4.4) |
| Refinement | ||||
| Resolution (Å) | 50.0-3.1 | 50.0-3.1 | 50.0-3.1 | 50.0-3.1 |
| No. unique reflections |
1,116,606 | 1,087,126 | 1,105,502 | 1,090,696 |
| Rwork/Rfree | 23.7/26.4 | 24.7/28.5 | 23.8/27.5 | 24.3/26.7 |
| No. atoms | ||||
| RNA | 205,448 | 205,202 | 205,200 | 205,442 |
| Protein | 100,888 | 100,888 | 100,888 | 100,888 |
| B-factors | ||||
| RNA | 99 | 82 | 90 | 76 |
| Protein | 103 | 86 | 92 | 81 |
| R.m.s deviations |
||||
| Bond lengths (Å) |
0.008 | 0.008 | 0.008 | 0.007 |
| Bond angles (°) | 1.2 | 1.3 | 1.3 | 1.2 |
*
I/σI =1.97 at 3.2 Å resolution
†
I/σI =2.08 at 3.2 Å resolution
§
I/σI =1.92 at 3.2 Å resolution
‡
I/σI =2.58 at 3.2 Å resolution