Table 1.
Primer pair sequences and allele distribution of 10 microsatellites among clone A, clone D and clone A+ of gibel carp*
| Locus (YJ) | Primer pair sequence | Annealing tempreture | Allele size (bp) | ||
|---|---|---|---|---|---|
| Clone A | Clone D | Clone A+ | |||
| 0001 | 5'-CTGGCATGAAGACTGGCTC-3' 5'-CAACAACACATATCAGCTCC-3' |
53°C | 84, 94, 100 | 88, 92, 96 | 84, 94, 100 |
| 0003 | 5'-TGAAGTTATTAGAAAGAGAG-3' 5'-CTTGATGATGTCTATGTGTG-3' |
53°C | 270, 288, 302 | 260, 290, 292 | 270, 288, 302 |
| 0004 | 5'-CATAGAGGCGTTTCATAGAG-3' 5'-CAGATAAATACAGTAAGCCA-3' |
50 - 55°C | 210, 220, 224 | 218, 222, 226 | 210, 220, 224 |
| 0005 | 5'-TAATAAGGTACATAGTCATAG-3' 5'-GTCAGCCTCCACCACGAATC-3' |
50 - 55°C | 226, 230, 234 | 222 | 226, 230, 234 |
| 0033 | 5'-CGGACACAAGAACGCCAAC-3' 5'-GGACTGGGCTGAAACTGATG-3' |
50 - 55°C | 172, 178 | 176 | 172, 178 |
| 0039 | 5'-GAAGAATACTTTATGACTGAGG-3' 5'-GACCAAGACAGACAGCCCAG-3' |
50 - 55°C | 138, 150, 156 | 136, 138, 156 | 138, 150, 156 |
| 0042 | 5'-GGCCACCTACAGTATATGC-3' 5'-GAAAACCAGGACCGACATG-3' |
50 - 55°C | 110, 114, 116 | 110, 112, 118 | 110, 114, 116 |
| 0040 | 5'-CCAGTATTAGGGAGCGTTC-3' 5'-GTTTCGTCTTCACAATCAGAA-3' |
50 - 55°C | 124, 138 | 132, 148 | 124, 138 |
| 0010 | 5'-GATGGTTGTGCTGTGAGCT-3' 5'-GAGTTCGTTTACATCTGGAC-3' |
53°C | 150, 156, 166 | 152, 160, 162 | 150, 156, 166 |
| 0022 | 5'-CACCAACTTTAGGCACATTTG-3' 5'-CCAGACTCCCACGTCATG-3' |
53°C | 144, 156 | 140, 162, 170 | 144, 156 |
| Total | 10 | 27 | 25 | 27 | |
*For each clone and each generation, 10 individuals were sampled and analyzed, and clone A+ samples of three generations, such as the third, fifth and seventh generation, were examined. No any individual difference was detected within each clone and among different generations.