Figure 6. K5 WT-THP-1 cells are more responsive to Flt-3 and PDGF-ß-induced signaling and have increased Erk activation.

Equal numbers of vector- and K5 WT-expressing THP-1 cells were serum starved overnight and stimulated with (A and C) Flt-3L (40 ng/ml) or (B) PDGF-ß (20 ng/ml) for ten minutes after which cells were washed and then treated with pervanadate for ten minutes at the times indicated. Western blot (WB) was performed on normalized whole cell lysates using (A and B) anti-phospho-tyrosine (4G10) (pTyr) or (C) anti-phospho-Erk antibodies. For panels A and B, 10 ug of THP-1 K5 WT lysate and 30 ug of THP-1 lysate were loaded to make comparison of bands easier between the two cell lines. Equal amounts of each lysate, 30 ug/lane, were loaded for panel C. Blots were re-probed for actinin (A and B) or total Erk (C). Data are representative of three independent experiments.