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. 2011 Apr 7;6(4):e18557. doi: 10.1371/journal.pone.0018557

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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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A. CASZ1a and CASZ1b transcript structure (top). Exon 16 is differently spliced to produce these two transcripts; CASZ1a and CASZ1b protein structure (bottom); CASZ1b lacks the C-terminal region of CASZ1a, which includes 6 zinc fingers. B. mCASZ1a and mCASZ1b mRNA level in neuronal tissues were detected by real time PCR using mCASZ1a and mCASZ1b specific primers. The two isoforms are dynamically expressed in the nervous system during development with mCASZ1a having a relatively higher level of expression compared to mCASZ1b. C. The ratio of mCASZ1a and mCASZ1b mRNA varied during development in different regions of the brain: a. Medulla; b. Frontal, Posterior, Enthorithinal cortex and Hippocampus; c. Hypothalamus, Olfactory bulb; d. Midbrain, Spinal cord, Thalamus, Pons and Striatum.