Figure 5. Quantitative assessment of the silencing efficiency of ASON-Bulge-DNAzyme by GFP fluorescence assay.

BHK21 cells were harvested and lysed in 200 µl lysis buffer (0.1 M Tris-HCl, 0.1% Triton X-100, 2 mM EDTA, pH 7.8). Thefluorescence of eGFP was measured using a luminometer. The error bars denote Standard deviation (SD).