Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Apr 7;6(4):e18593. doi: 10.1371/journal.pone.0018593

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Banerjee et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

(A) Expression and purification of mASAL in 15% SDS-PAGE analysis; lane 1 represents MBP fusion protein; lane 2 represents fusion protein digested with Factor Xa; lane 3 represents hydroxyapatite column purified mASAL; lane 4 represents Western blotting of purified mASAL against an anti-ASAL antibody showing a band at 12.5 kDa; lane M represents a standard protein molecular weight marker. (B) The corresponding elution profiles from size exclusion chromatography results of the mutant protein from a Biosep-SEC-S-2000 column of Phenomenex at the flow rate 2 ml/min. The figure shows fused mASAL with MBP (pick at 3.5 min), MBP and mASAL after factor Xa digestion (picks at 4.8 min and 6.15 min, respectively) and pure mASAL (pick at 6.1 min).