Table 1. List of primers used in stepwise PCR amplification for replacement and insertion mutation in ASAL.

PCR number	Primer combination	Primer sequence
1st PCR	Primer 1	5′AGCTGGATCCATGGCCAGGAACCTACTGACGAACGGTGA 3′
	Primer 2	5′ATTATCGTAAATGACAACGTTC 3′
2nd PCR	Primer 1	5′AGCTGGATCCATGGCCAGGAACCTACTGACGAACGGTGA 3′
	Primer 3	5′AGACCAAATCGCATTATTAGA 3′
3rd PCR	Primer 1	5′AGCTGGATCCATGGCCAGGAACCTACTGACGAACGGTGA 3′
	Primer 4	5′AACCTAGGTACCAGTAGACCAAATCGC 3′