Figure 5. Drosophila Gα_s is localized in the sensilla of the olfactory sensory neurons.

(A) Alignment of C-termini of Gαs from different species showing the high degree of conservation in this part of the protein. (B) RT-PCR with RNA prepared from manually collected antennae using Gα_s specific primers (+ RT), control lane is RNA without reverse transcription (-RT). (C) Western blot of manually collected antennae probed with anti-Gα_s showing a band approximately 50 kD, which disappears after pre-incubation of the antibody with a specific blocking peptide. (D) Staining of the third antennal segment using Gα_s specific antibodies. Red - Gα_s; Green – mCD8-GFP (Or83b-Gal4; UAS-mCD8-GFP).