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. 2011 Apr 7;6(4):e18586. doi: 10.1371/journal.pone.0018586

Figure 2. Differential bleaching kinetics of CFP and YFP in different fluids.

Figure 2

293 cells were transfected with either CFP (A) or YFP (B) and the bleaching of the fluorescent proteins was recorded either for live cells in medium or for fixed cells after mounting in Fisher Scientific Ultramount (Ultramount) or Dako Fluorescence Mounting Fluid (Dako). Bleaching was recorded on a Zeiss Axiovert 135 microscope using continuous illumination at the respective wavelength with 50% energy of a 100W Mercury-lamp. Metamorph™ 7.5 software was used to acquire images at 5 sec intervals. Camera: PhotometricsCoolsnap; exposure time: 50 msec. Data points are mean of n = 4. Error bars represent standard error of mean (SEM).