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. 2011 Apr 7;6(4):e18645. doi: 10.1371/journal.pone.0018645

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Deplagne et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Sections from WT (a, c) and nm23-M1^−/− (b, d) nipples were sectioned and stained by hematoxylin and eosin (a, b) or trichrom Masson (c, d). The general architecture of dermis and epidermis of the mutant nipple doesn't seem overly changed. However, close examination revealed that the final opening of the lactiferous canal is obstructed by epithelial cells in the mutant glands (arrow). M: milk. Original magnification X200 (a, b) X400 (c,d).