Skip to main content
. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: J Cell Physiol. 2011 Jul;226(7):1828–1842. doi: 10.1002/jcp.22514

Figure 2. RT-PCR analysis of functional expression of TRPV channels in cultivated human corneal epithelial cells (HCEC).

Figure 2

Conventional RT-PCR revealed TRPV1, TRPV2 and TRPV4 gene expression in cultivated HCEC. Resulting PCR products (TRPV1 285 bp, TRPV2 228 bp and TRPV4 419 bp) were visualized in ethidium bromide-stained 2% agarose gels. PCR control (Ø) was performed without reverse transcriptase during cDNA synthesis to exclude primer binding to genomic DNA. As positive control (C), cDNA from human brain was used. M, DNA Ladder. HCEC cDNA integrity/stability was validated based on invariant and level of expression of β-actin as a housekeeping gene (standard lab protocol).