Table 1.

Quantification of morphological changes caused by myc-SH3A expression in cultured ECs

Experimental condition	Abnormal caveolae clusters open to the apical side of EC	Apparently normal – omega-shaped caveolae	Single caveolae with staining dense rings around the neck	Caveolae with elongated necks
Control ECs	3 ± 0.4	82 ± 8	2 ± 0.3	8 ± 0.9
myc-SH3A transfected ECs	30 ± 5.3	15 ± 6.1	38 ± 4.2	34 ± 2.5

Fifty electron micrographs (30,000× magnification) per experimental condition, containing ECs profiles of control and myc-SH3A transfected ECs were used to count the number of (i) abnormal caveolae clusters open to the apical side of the endothelial PM, (ii) apparently normal caveolae, omega shaped, connected to the PM either directly or through short necks, (iii) caveolae with their neck surrounded by staining dense collars and (iv) caveolae with elongated necks (less than ½ of caveolar diameter). Quantification of these morphological characteristics and the measurement of the length PM on the electron micrograph used were performed as described previously [10, 28]. The data were normalized per 100 μm length PM and are averages ± S.D. of three independent experiments.